human blca cell lines umuc3  (ATCC)

Journal: Cell & bioscience

Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors.

doi: 10.1186/s13578-025-01379-w

Figure Lengend Snippet: Fig. 1 OBSCN expression in pan-cancer analysis. (A) The expression of OBSCN in tumors and adjacent tissues were revealed in pan-cancer analysis; (B) Pan-cancer analysis of OBSCN expression in tumor cell lines; (C) tSNE degradation and visualization analysis of OBSCN expression in single-cell sequenc ing database GSE135337; (D–E) Expression levels and proportions of OBSCN among six types of cells in TIME of BLCA annotated with R package SingleR

Article Snippet: Cell culture and Si-RNA transfection Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing

Journal: Cell & bioscience

Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors.

doi: 10.1186/s13578-025-01379-w

Figure Lengend Snippet: Fig. 3 OBSCN deficiency promoted the progression of BLCA. (A) The mRNA expression of OBSCN was stably down-regulated after knockdown; (B) The protein expression of OBSCN was stably down-regulated after knockdown; (C) CCK8 experiment: There was no effect on the proliferative ability of BLCA cells after OBSCN knockdown; (D) Scratch test: The invasion ability of BLCA cells was enhanced after OBSCN knockdown; (E) Transwell experiment: The migration ability of BLCA cells was enhanced after OBSCN knockdown; (F) The results of pathways enrichment were analyzed by GSVA between low and high OBSCN expression of BLCA samples; (G) The results of pathways enrichment were analyzed by GSEA between low and high OBSCN expression of BLCA samples; (H) OBSCN knockdown enhanced the EMT of BLCA cells

Article Snippet: Cell culture and Si-RNA transfection Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing, Stable Transfection, Knockdown, Migration

Journal: Cell & bioscience

Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors.

doi: 10.1186/s13578-025-01379-w

Figure Lengend Snippet: Fig. 5 OBSCN expression predicted the efficacy of PD-L1 ICIs in BLCA. (A). The relationship between OBSCN expression and OS in the IMVigor210 cohort; (B). Correlation between OBSCN expression and clinical response in the IMVigor210 cohort; (C). Correlation between OBSCN expression and molecular subtypes in the IMVigor210 cohort; (D). Correlation between OBSCN expression and CD8+T effector score in IMVigor210 cohort; (E). The relationship between OBSCN expression and OS in the GSE176307 cohort; (A) Correlation between OBSCN expression and clinical response in the GSE176307 cohort

Article Snippet: Cell culture and Si-RNA transfection Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing

Journal: Cell & bioscience

Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors.

doi: 10.1186/s13578-025-01379-w

Figure Lengend Snippet: Fig. 6 Potential mechanism of OBSCN expression deficiency in the immune evasion of BLCA. (A) Heatmap representing the correlation between OBSCN expression and chemokines in pan-cancer analysis. (B) Heatmap representing the correlation between OBSCN expression and infiltration of CD8+T cells in pan-cancer analysis from TIMER2.0 website. (C) IHC staining of CD8A on the TMAs of Shuguang cohort. (D) IHC analysis of the CD8+T cells between OBSCN low and high group in Shuguang TMAs (n = 80). (E) IHC staining of PD-L1 on the TMAs of Shuguang cohort. (F) IHC analysis of the PD-L1 expression in BLCA between OBSCN low and high group in Shuguang TMAs (n = 80). (G) WB test was used to evaluate the expression of PD-L1 after knocking down OBSCN in BLCA cell lines UMUC3 and 5637

Article Snippet: Cell culture and Si-RNA transfection Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemistry