Sequencing:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Expressing:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Immunohistochemistry:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Western Blot:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Quantitative RT-PCR:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Staining:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Migration:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
In Vitro:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
CCK-8 Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Colony Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
EdU Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Knockdown:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Wound Healing Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Transwell Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Flow Cytometry:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
RNA Sequencing:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Software:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Transwell Invasion Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Inhibition:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Phospho-proteomics:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Liquid Chromatography with Mass Spectroscopy:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Mass Spectrometry:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Co-Immunoprecipitation Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Proximity Ligation Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Pull Down Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Incubation:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
SDS Page:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Translocation Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Binding Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
ChIP-qPCR:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
In Vivo:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Over Expression:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Control:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Luciferase:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Reporter Gene Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Activity Assay:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
RNA Expression:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Zeta Potential Analyzer:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Microscopy:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Imaging:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Immunofluorescence:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
Stable Transfection:Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer.
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer
Article Snippet: We purchased human uroepithelial cells SV-HUC-1 and human BLCA cell lines UMUC3, HT1197, SW780, J82, T24 and 5637 at the American Type Culture Collection.. Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).Culture media specifications were standardized as follows: 5637 in RPMI-1640; SW780 in L-15 Medium; SV-HUC-1 in F-12 K Medium; UMUC3/HT1197/J82 lineages in Eagle’s Minimum Essential Medium; T24 populations in McCoy’s 5a (all media reagents were sourced from Invitrogen, USA).
Article Title: Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors
Article Snippet: Human BLCA cell lines UMUC3 and 5637 were obtained from American Type Culture Collection (ATCC).. All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).All cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco).
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